Creative Enzymes Provides Enzyme Expression and Purification Service

Genetic engineering improves enzyme protein expression.

Genetic engineering means that DNA is recombined in vitro to obtain a suitable DNA fragment and then put into the proper host cells for expression. By changing the genetic elements and regulatory elements of the gene, the enzyme expression can be changed.

Typical bacteria and fungi used to produce enzymes have 2 to 10000 related genes. The gene encoding the protein of Bacillus subtilis is located on a long chain of chromosome DNA containing 4 million 200 thousand base pairs. Aspergillus niger contains about 35 million base pairs encoding the enzyme protein, which are located on the long chain of the 8 DNA chromosomes. A gene determines the amino acid composition and specific amino acid sequence of a protein. A typical fragment of a gene encoding an enzyme produced by a bacterium is about 1000 nucleotides in length and encodes a protein of about more than and 300 amino acids. In addition to the DNA fragment encoding the gene, there is a DNA fragment that controls the expression of a gene called a promoter. Only a fraction of the genes in an organism are always in expression. The promoter element controls the coordinated expression of the corresponding gene fragments, and the function of the DNA promoter fragment can be compared to a light switch, which controls the initiation, the expression and the termination of the corresponding structural gene.

Genetic engineering also requires the transfer of recombinant DNA into the appropriate host cells. Enzyme Expression in E. coli and Bacillus subtilis are relatively easy.

Method for enzyme production:

Extraction method

By using various techniques, the enzyme is extracted directly from the plant cells or tissues. The extraction method is simple and easy, but it is limited by the source of raw materials.

Chemical synthesis

It was a new technology in the mid 1960s.

Only those known chemical structure enzymes can be synthesized; the cost is relatively high.

It is still in the stage of laboratory synthesis.

Microbial fermentation

It is the main method of producing enzyme since 1950s.

The method of synthesizing the required enzymes by the activity of microbial cells is microbial fermentation.

Enzyme fermentation is the main method of enzyme production.

Creative Enzymes provide a series of enzyem services to serve customers, including gene cloning, expression, and optimization&purification of enzymes.

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Company Name: Creative Enzymes
Contact Person: Jack Forst
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Phone: 6316197922
Address:45-1 Ramsey Road
City: Shirley
State: New York
Country: United States

Full Length cDNA Clones Lauched by Creative Biogene

The full-length ORF clone contains only the coding sequence of the full-length protein, while the other full-length cDNA clones contain some untranslated sequences, such as the 5’side or 3′ side non translation. It is well known that these untranslated sequences may have a negative effect on the transcription and translation processes of the encoded proteins in the host.

If it is the ORF expressed clone, it can be transfected into cells and expressed in cells.

In the general situation, the carrier of cDNA clone is not the expression vector, so it can not be directly used for transfection of cells.

The difference between ORF, cDNA and CDS:

1.what is a full-length ORF clone?

A full-length ORF clone is a plasmid inserted into a DNA fragment encoding a full-length protein. The inserted DNA fragment only contains sequence incoding a full-length protein, and does not contain the untranslated region of 5 ‘or 3’ end (UTRs) or intron.

2.why should the full length ORF cDNA clones are used instead of other full-length cDNA clones?

The full-length ORF clone contains only the coding sequence of the full-length protein, while the other full-length cDNA clones contain some untranslated sequences, such as the 5’side or 3′ side non translation. It is well known that these untranslated sequences may have a negative effect on the transcription and translation processes of the encoded proteins in the host.

ORF sequence: open reading frame. It is suggested that the DNA sequence may be a gene, but the specific gene coding sequence requires additional content. If the promoter and terminator sequence of the DNA sequence are identified, the amino acid information of the sequence can be identified.

cDNA sequence: complementary DNA sequence. It refers to the process mRNA forms DNA under the action of reverse transcriptase. This DNA is cDNA sequence. It has no difference between introns and exons. In prokaryotes, it can be used as a gene sequence encoding polypeptide, but in eukaryotes, due to lack introns, so it should not be used as a gene sequence encoding polypeptide.

CDS sequence: coding sequences, which contain sequences from the start codon to the stop codon.

In order to facilitate the search and query, Creative Biogene tries to improve clones construction and describe these cDNA clones to provide customers with the best quality and fastest service!

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Company Name: Creative Biogene
Contact Person: Wendy Wilson
Email: Send Email
Phone: 6316197922
Address:45-1 Ramsey Road
City: Shirley
State: New York
Country: United States

Creative Biolabs Designs and Constructs CAR Molecule Vectors

CAR-T design is based on similariry of structure in Ig and TCR, simulating the variable region of TCR alpha and beta chain by single chain variable fragment (scFv) , through the identification of tumor specific antigen, directly transmitting signal to embedded artificial immune receptor tyrosine based activation sequence on a single polypeptide chain signal, and activating the intracellular Akt signaling pathway, thereby activating T cells and killing tumor cells.

CAR-T cells specifically recognize specific antigens on the surface of any tumor cell, such as proteins, carbohydrates and glycolipids, which do not require antigen processing and presentation.

After nearly more than 10 years of research and exploration, CAR-T’s design has developed with several versions. The most popular recent technologies include the enhanced 4th generation CAR-T and the universal fifth generation CAR-T technology. However, most of the current clinical trials use the second generation (single costimulatory molecules) and the third generation (double costimulatory molecules) CAR-T design. The chimeric antigen receptor is composed of four parts: extracellular specific antigen recognition region, hinge region, transmembrane region and intracellular signal transduction domain. Therefore, the basic design of CAR is also a combination of these four parts optimization.

The first generation CAR-T cells fuse immunoglobulin scFv and Fc RI receptor or CD3 complex intracellular domain to form chimeric receptor; the second generation CAR-T cells add activation signals, such as CD28, OX-40 and 4-1BB on the chimeric antigen receptor, in order to improve the cytotoxicity and proliferation of T cells and prolong the survival time of T cells. Based on the understanding of the activation mechanism of T cells, CD28 molecules can provide second activation signals and further enhance the TCR/CD3 signal; the third generation CAR-T cell adds a co-activated signal on the basis of the second generation.

CAR T construction services are as follows:

Creative Biolabs team has long been engaged in the research of CAR-T, like the space structure of CAR molecules, costimulatory factor and other attributes, which can optimize the project according to the customer.

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Company Name: CAR-T
Contact Person: Bella Smith
Email: Send Email
Phone: 6316197922
Address:45-1 Ramsey Road
City: Shirley
State: NY
Country: United States

Austracoat warns homeowners about the dangers of lead base paints

Melbourne’s Leading Commercial Painters, Austracoat, admonishes homeowners and the public on the dangers of using lead base paints and how to reduce exposure to the dangerous substance.

Austracoat, one of Australia’s leading painters recently brought to the notice of homeowners and parents, the dangers of lead base paints and why they should be avoided as much as possible. The painting giant also shared ways of reducing exposure to lead, to ensure that the home and environment is as safe as possible. It is, however, disturbing that most homeowners across Australia and even clients of Austracoat are not aware of the dangers of lead-based paints and this is a major cause for concern.

The incidence in cases of health problems associated with the exposure of lead is increasing with different cases recorded across the globe, and Australia has not been immune to such situations. In 1991, it was described as the number one environmental threat to the health of children in the United States, making it a major concern for parents and guardians.

Lead-based paints can be found in different places in the home, which include window frames, outside the homes, walls and other such surfaces, with exposures created due to improper removal of the paints from surfaces.

Different health hazards have been attributed to exposure to lead, and study has shown that lead can affect practically all systems within the body, with the effects dependent on the level of exposure. Coma, convulsions, and death are some of the effects of being exposed to lead at high levels. Exposure to lead at lower levels can have an adverse effect on the brain, blood cells, kidneys and the central nervous systems. These are some of the reasons why they should avoid being exposed to lead.

Young children and fetuses are usually the worst hit with lead exposure, and some of the effects of such exposures include delays in mental and physical development, increased behavioral problems, shortened attention spans and lower IQ levels. Fetuses, children, and infants are also more vulnerable as their body easily absorbs the lead, and their infant body system makes the effect more damaging.

There are different ways of reducing one’s exposure to lead, especially as regards lead-based paints. Some of these ways include keeping areas where children play as clean and dust-free as possible, seeking professional help when removing lead-based paints, eating right, and more importantly, reducing the risk from lead-based paint.,q_auto,f_auto/message_attachements/378513551/original/leadpaint2.jpg

Austracoat in a bid to ensure that houses are safe and inhabitants are not exposed to lead, use state-of-the-art equipment and tools, and carefully prepares the home by covering floors to ensure that no part of the unwanted paint is left in the home. Austracoat also uses dust extracted Sanders for painting the home and other such facilities.,q_auto,f_auto/message_attachements/378513441/original/Leadpaint1.jpg

About Austracoat

Austracoat is one of Australia’s leading commercial painting companies. Headquartered in Melbourne, Victoria, Austracoat has been operating for more than ten years, servicing several commercial businesses and individual across Melbourne.

Austracoat boasts of highly trained and professional staff, using state-of-the-art equipment to cater for any project regardless of the size or location within Melbourne. Services offered by Austracoat include commercial painting, protective coating, and long-term facility maintenance.

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Company Name: Austracoat
Contact Person: Narre Warren
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Phone: (03) 9704 8046
Address:16 Boston Court
City: Victoria
State: Melbourne
Country: Australia

Pin-Up Girls: An Initiative by a Woman for the Women

An Inspiring Initiative by an Australian Woman, Caitlin who is Creating a Pin-Up Book Filled with Women who are all Different Shapes, Sizes and Nationalities.

An Australian Woman, Caitlin has announced that she will be helping women from around the world to gain their self-confidence back by creating a pin-up book called ‘Pin-Up Girls.’ She is seeking support from around the world to complete this dream and she is using Kickstarter to back this project. Her goal is to raise $65,000 Australian Dollars through this Kickstarter campaign to crowdfund this amazing initiative and the campaign will be active till April, 19th 2017. Caitlin has experienced severe situations and she hopes that she will be able to help women and prevent them from experience the pain she has been through.

The book, Pin-Up Girls will feature women who are or have been bullied about their size, face, body, nationality, sexual orientations or their physical disabilities, etc. Such women lack self-confidence and Caitlin hopes to restore it through this amazing initiative. It will make them feel good about themselves and will spread a message of positivity and strength. The book is all about self-image and shaping thoughts to shape the life and future of those women who have been bullied. Such initiative has never been taken before and it will help the women not only from Australia but from around the world to be strong again.

Funds raised through this Kickstarter campaign will help Caitlin to pay the professional photographers and makeup artists as well as space to hire along with the clothes, accessories and just general costs like equipment and travel, etc.

Generous contributors can back this project by accessing the following link:

About Pin-Up Girls

Pin-Up Girls is an initiative by a brave Australian woman, Caitlin who has made it her mission to support the women who have been bullied due to their appearance, shape, size, disabilities or any other issue they have been going through. The book will strengthen the women around the world and will give them a courage to stand up for their rights. The author is crowdfunding this campaign to turn this amazing project into reality and she is now seeking public support for this great initiative.

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Company Name: Pin-Up Girls
Contact Person: Caitlin Guthrie
Email: Send Email
Phone: 0487408806
City: Burnie
Country: Australia

Timeshare Pro Plus Wins Best Strategic Partner at the 2017 Perspective Magazine Awards

Orlando, Florida – February 28, 2017 – Timeshare Pro Plus, producers of the only timeshare-specific, online title and back office related services software, took home the Perspective Magazine Award for Best Strategic Partner at the GNEX Conference in February.

Timeshare Pro Plus’ software modules are fully integrated with DocuSign®, the top providers of electronic signatures and leading solutions for Digital Transaction Management. The software is setting a new standard for convenience and ease through the integration for all businesses and users. The unification of Timeshare Pro Plus and the electronic signature service is a strategic partnership that assists both resorts and customers by helping organizations of all sizes and industries accelerate contracts, approvals, and workflows.

Winners were decided by a format unique to the timeshare and hospitality industry, with four anonymous veteran industry judges and an online voting portal where industry professionals had their opportunity to influence the outcome. Contenders shared their nomination through marketing materials, company websites, and social media platforms. Each individual judge’s vote counted for 20 percent of the results, with online voting accounting for the remaining 20 percent of the score.

“We are proud to offer services that combine our modules with DocuSign®, and winning the Perspective Magazine Award for Best Strategic Partner is a testament to how we’re impacting the direction of the industry,” says Dave Heine, President of Timeshare Pro Plus. “Just as we did at GNEX, Timeshare Pro Plus will be exhibiting at ARDA and we’re excited to generate new business with partners who are backed by the confidence that this award conveys.”

Now taking only minutes, the back and forth inconvenience of mailing or faxing documents has come to an end with the integration thoroughly eliminating hassle, cost, and most importantly lack of security. Timeshare Pro Plus the perfect platform to for business solutions and is clearly deserving of the 2017 Perspective Magazine Award for Best Strategic Partnership.

The addition of DocuSign® replaces printing, faxing, scanning and overnighting documents with the easiest, fastest, most trusted way to make every approval and decision digital. Timeshare Pro Plus modules offer the electronic signature solution and Digital Transaction Management platform to help clients send, sign and manage contracts from anywhere, anytime, on any device. Timeshare Pro Plus resort clients will now be able to offer their customers an automated method of signing contracts and other documents. 

Timeshare Pro Plus offers online software for resort developers, management companies, HOAs, and sales organizations to use when handling the paperwork and communications activities involved in the process of transferring a title. Modules including,, and may be used individually or as a whole package to complete necessary forms, collect payments and fees, and produce documents in less than an hour.

About Timeshare Pro Plus

Timeshare Pro Plus uses the latest cloud technology to save its clients time, paper and postage. The latest HIPPA Compliant security measures maintain the highest level of security required to handle sensitive information online for resort developers, management companies, HOAs and sales organizations when handling
the paperwork and communications activities involved in the process of transferring a title. The software is capable of simplifying the process for both resorts, timeshare buyers and sellers. Depending on clients’ particular needs or requests, Timeshare Pro Plus offers several modules, including including,, and, that may be used individually or as a whole package to complete necessary forms, collect payments and fees, and produce documents in less than an hour.

For more information, visit

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Company Name: INTUITION Brand Marketing
Contact Person: Danielle Barnes
Email: Send Email
Phone: 407-730-3565
Address:4700 Millenia Blvd, Suite 370
City: Orlando
State: Florida
Country: United States

Fine Art Nature Photographer Aaron Reed Celebrates Successful 2016 Doubling Sales for 4th Year in a Row

Aaron Reed, a fine art nature photographer who makes his work available worldwide as large limited edition large acrylic prints, couldn’t be more pleased with the ever growing interest in his work. Reed recently announced 2016 was his most successful year yet, not only selling out of his first limited edition piece “Living Lightning”, but also doubling 2015’s sales.

February 28, 2017 – There’s no doubt at all that the right choice in a fine art photographic print can transform a room or even an entire home or office in a very real, dramatic way.  A rising force in this area is the in-demand fine art nature photographer Aaron Reed

In an exciting news, Reed recently celebrated a wildly successful 2016 – doubling sales of his large limited edition acrylic prints for the fourth year in a row, in addition to completely selling out his limited edition  print “Living Lightning”.

“2016 has completely exceeded even my highest expectations,”  commented Reed.  “It’s a wonderful feeling to have so many people appreciate my work and honor me by putting the prints in their homes or offices.  I’m looking forward to seeing what 2017 brings, since the year is already off to a great start.  None of this would be possible without my valued customers and supporters.”

According to Reed, the photographer specializes in fine art landscape and nature photography.  He makes his work available through his own Aaron Reed Luxury Nature Photography website, which not only shows his growing catalog, but also allows customers to place orders for his prints directly.  The fine art prints are created with various photographic papers, face-mounted Lumachrome acrylic prints and High Definition aluminum ChromaLuxe panels, with the end result being quite remarkable highlighting Reed’s creative use of lighting, attention to detail and choice in subject.

Reed’s limited edition collection highlights what he considers the best of his work, and depending on the piece limits the number of prints to 50, 100 or 200 prints sold with the guarantee the piece will never be produced again as a fine art print.  All are signed and numbered and come complete with a certificate of authenticity. 

Reviews of Aaron Reed Luxury Nature Photography have been completely positive across the board.

A recent customer remarked in a five star review, “Breathtakingly beautiful photographs! The detail and depth is amazing! I am beyond pleased.  Thank you!”

For more information be sure to visit

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Company Name: Aaron Reed Photography
Contact Person: Aaron Reed
Email: Send Email
Phone: 425-239-0842
Country: United States

Marketing1on1 Releases Newly Re-Designed Website

February 28, 2017 – The website for Marketing1on1 has recently been re-designed. The new design is a true showcase of the appealing design efforts that Marketing1on1 can provide to people aiming to get the most out of their internet marketing needs. This especially comes as the site is offering an extensive variety of solutions for the marketing demands that people hold.

Marketing1on1 offers web development solutions among its many internet marketing services. The development process works with a carefully orchestrated series of steps including identifying what key points on a site the client wants to utilize and how individual sections are to be developed. The efforts provided by Marketing1on1 are extensive and rich.

The development process is organized to create a visually appealing site. The company especially places emphasis on offering sites that are easy to load up, thus making them easier for people to read.

Marketing1on1 is also promoting the many other internet marketing services it has through its redesigned website. These include the search engine optimization features and link building services that the company is known for. Content writing services are also available with an emphasis on helping to produce news and press release documents.

Other services available from the company include Google Places optimization, social media support and paid per click advertising services. Clients are encouraged to talk with Marketing1on1 to get more information on what they can utilize when it comes to getting more out of their marketing needs.

The efforts being managed by Marketing1on1 are especially being highlighted as solutions to help with getting the most out of one’s business needs. The newly redesigned site for the company is a clear sign of what makes the system so appealing.

Quick quotes are available through the service. People can get quick quotes online by entering in one’s name, email, and information on what has to be managed. The information will be reviewed by a Marketing1on1 to make it easier for someone’s needs to be managed properly. This can especially make a difference when reviewed carefully enough. Proper individualized consultations will be provided to each person who asks for support in any form.

About the firm:

Marketing1on1 is a website that provides online marketing services for businesses. The services include solutions dedicated to improving search engine rankings while also making sites more visible and easier to read.

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Company Name: Marketing1on1
Contact Person: Public Relations
Email: Send Email
Phone: 818-538-4805
Country: United States

Local Nonprofit Charity – Houston Birthing Connection gets proud sponsorship from Kenneth R. Henry – The Movement Agency LLC.

Houston, Texas – Houston Birthing Connection (HBC) is a nonprofit 501 (C) (3) charity organization that has dedicated its platform to serving the underserved society. HBC has received a generous sponsorship by The Movement Agency LLC., a large organization dealing with Commercial and Personal Lines insurance and other policies. The organization has made a commitment to help serve the community and enable it to become a better living place for every person, despite their social status, caste or creed.

This sponsorship will allow Houston Birthing Connection to enhance their efforts of serving the community. Since the organization plays an important role in helping middle and high school students manage their life, studies and parental duties, this sponsorship will be directed towards a number of charity initiatives that Houston Birthing Connection has undertaken. The two founders are providing opportunities for a better life for members of the community. We as a community have become so involved in our own businesses, which we forget to look around at how others are spending their lives. A society that shares each other’s sorrows and difficulties grows together into a more nourishing and prosperous community. It enables every person to live a better life, garner quality education and raise upstanding members of the society. The sponsorship will provide finances that will allow Houston Birthing Connection to ensure this happens.

About Housing Birthing Connection:

Housing Birthing Connection is a nonprofit organization in Houston, Texas and its surrounding areas. This organization aspires to provide assistance to middle and high school students, who have been bestowed the responsibility of young children or currently pregnant. The organization provides education and support as well as emotional assistance to cope with the challenges that come when taking care of such young minds.  Adolescent parents, who are also students, have to take part-time jobs in order to support themselves and take care of their children. This keeps them from spending time with their kids and also causes them to lose focus on studies. By helping them and empowering them to reshape their thinking, Houston Birthing Connection ensures that they give time and attention to their education and give quality time to their young ones.

About the Movement Agency LLC.:

In Houston, insurance is normally handled and owned by policyholders. These agents can help you with a number of situations relating to commercial and personal insurance. The Movement Agency LLC is one of the leading organizations with Nationwide Insurance, which provide a full range of insurance and financial services across the country. These insurances include business insurance, automobiles, motorcycles, boats, insurances for homeowners, for pet owners, farmers, life and commercial insurance.

Moreover, the organization also provides other useful services in administration such as annuities, mortgages, mutual funds, pensions, long-term savings plans and specialty health services. Kenneth Henry understands the difficulties related insurance and how confusing and complicated it can get. That is why their professionals are available to assist you with the process. Whether you need advice or just require answers to questions, this company treats their clients like family and will assist you in the best possible manner.

Having a knowledgeable agent can save you a lot of money, time and energy, and Kenneth R. Henry is the best one on the market.  He can be contacted at 713-987-0005 or emailed at [email protected]

If you or your business would like to join forces in the community with Houston Birthing Connection please contact Tie at (832) 308-0776 or email the office.

All donations are tax deductible.

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Company Name: Houston Birthing Connection
Contact Person: Tieasha Waddy
Email: Send Email
Phone: (832) 308-0776
Address:3116 Telge # F
City: Houston
State: Texas
Country: United States

Process Analytical Technology Market worth 3.30 Billion USD by 2021

On the basis of products and services, the process analytical technology market is segmented into analyzers, sensors and probes, and samplers. The products segment is expected to account for the largest share of the market in 2016. Increasing drug discovery and development activities and stringent regulations for drug safety are stimulating the demand for PAT products in the market.

The report Process Analytical Technology Market by Technique (Spectroscopy, Chromatography), Measurement (On-Line, In-Line, At-Line), Products and Services (Analyzers, Probes & Sensors), End – User (Pharmaceutical Manufacturers, CRO & CMO) – Global Forecast to 2021″, provides a detailed overview of major drivers, restraints, challenges, opportunities, current market trends and strategies impacting the global market along with estimates and forecast of revenue.

The global process analytical technology market is poised to reach USD 3.30 Billion by 2021 from USD 1.77 Billion in 2016, at a CAGR of 13.3% from 2016 to 2021.

Market growth is primarily driven by factors such as government regulations on drug safety, need for manufacturing process efficiency leading to quality improvement, and technological advancements in analytical devices. Other factors supporting market growth include an increase in R&D spending by pharma and biopharma companies and increasing government investments in emerging countries. The emerging markets are also expected to offer plenty of growth opportunities for players in the market.

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The global process analytical technology market is segmented on the basis of technique, measurement, end user, products and services, and region. On the basis of products, the process analytical technology market for products is further divided into analyzers, sensors and probes, and samplers. The analyzers segment is expected to account for the largest share of the global market in 2016. Factors attributing to the growth of this segment include the high preference for molecular spectroscopy hand-held instruments that offer in-line as well as on-line analysis of the drug manufacturing process.

Based on measurement, the process analytical technology market is segmented into on-line, in-line, at-line, and off-line methods of analysis. The on-line segment is expected to account for the largest share of the process analytical technology market in 2016. On-line analysis is the most preferred method as it offers consistency and certainty in the results, owing to no human intervention, safety for operations due to automation, and a turn-around time of a few seconds. The method saves person-hours by automating the routine testing from laboratories and operators.

North America is expected to account for the largest share of the global process analytical technology market in 2016, followed by Europe. Stringent regulatory guidelines to provide safe drugs is encouraging the use of PAT and the presence of a large number of market players are propelling the growth of the North American process analytical technology market. Asia-Pacific region is expected to grow at the highest CAGR in the forecast period.

Growing government investments in pharmaceutical R&D and the rise in contract research and manufacturing organizations in emerging Asia-Pacific countries are the key factors driving the growth of the Asia-Pacific market.

The process analytical technology market is dominated by established players such as Thermo Fisher Scientific, Inc. (U.S.), Agilent Technologies, Inc. (U.S.), Danaher Corporation (U.S.), Bruker Corporation (U.S.), and PerkinElmer, Inc. (U.S.).

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Company Name: MarketsandMarkets
Contact Person: Rohan
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Phone: 1-888-600-6441
Address:701 Pike Street, Suite 2175
City: Seattle
State: WA
Country: United States